ABSTRACT A variety of methods to identify differences in gene expression exist, including subtractive hybridization, differential display, serial analysis of gene expression, and microarray hybridization. Some of these techniques have been used to investigate changes in gene expression in livestock, and important resources are being developed that will facilitate the use of others. The objectives of this review are to provide a general overview of techniques for evaluating gene expression in livestock and to identify resources currently available and in development for animal science researchers.
Implications
The evaluation of gene expression is an effective way to identify genes important in the regulation of traits of economic importance in livestock production. Several techniques have been developed for this purpose, and important resources are currently in development that will facilitate continued research and discovery in this area. The application of these research methods in animal sciences will contribute to a greater understanding of the genetic regulation of traits important to livestock production.
Key Words: Gene Expression, Laboratory Methods, Livestock, Techniques
© 2001, by the American Society of Animal Science. All rights reserved.
J. Anim. Sci. 2001. 79:E128-E135
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