Interpretive Summary: Characterization of sow milk N-linked glycoproteome over the course of lactation

By: Prabha Rajput, Uma K Aryal, Ketaki Bhide, Radiah C Minor, Sairam Krishnamurthy, Theresa M Casey

Milk is essential for healthy growth and development of neonates, with proteins in milk serving as key nutrients and regulators of these processes. Protein activity is affected by modifications made to their structure including the addition of sugar groups called glycans. Here we present the characterization of sow milk proteins modification with glycan groups on asparagine and glutamine amino acids in colostrum, transitional, and mature milk of pigs. We found 220 high confidences (found in at least two sows on one day) glycoproteins, and that the abundance of glycosylated proteins varied by stage of milk production and number of glycosylated sites.