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Interpretive Summary: Form of dietary selenium affects mRNA encoding cholesterol biosynthesis and immune response elements in the early luteal phase bovine corpus luteum

By: Benjamin R Crites, Sarah N Carr, James C Matthews, Phillip J Bridges

In regions with soils deficient in selenium (Se), producers should supplement this trace mineral to the diet of forage-grazing cattle. We previously reported that circulating concentrations of progesterone (P4) are affected by the form of Se supplemented to cows. In this report, we aimed to determine how the form of Se affects the transcriptome of the bovine CL, with the goal of elucidating form of Se effects on luteal steroidogenesis. Cows were supplemented with the industry standard, an inorganic form of Se, or a 1:1 mix of organic and inorganic forms (MIX), with corpora lutea recovered on day 7 of the estrous cycle. The effect of TRT (form of Se) on the luteal transcriptome was then evaluated using bovine gene 1.0 ST arrays (Affymetrix, Inc., Santa Clara, CA), with the results of the microarray analysis corroborated by targeted qPCR. Treatment altered >800 transcripts in the CL, including those regulating cholesterol biosynthesis and immune function. The effect of form of Se on luteal steroidogenesis appears to be the result of changes in cholesterol biosynthesis and uptake by luteal cells, with cross talk between immune and cholesterol regulatory pathways also apparent.

Read the full article on the Journal of Animal Science.